A Single Dose of Anti-HBsAg Antibody-Encoding mRNA-LNPs Suppressed HBsAg Expression: a Potential Cure of Chronic Hepatitis B Virus Infection

Author:

Chen Binfan1,Chen Yuchen2,Li Jian3,Wang Chunyu1,Song Wenping1,Wen Yumei1,Lin Jinzhong2,Wu Yanling1,Ying Tianlei1ORCID

Affiliation:

1. MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Engineering Research Center for Synthetic Immunology, School of Basic Medical Sciences, Fudan University, Shanghai, China

2. State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, China

3. Clinical Laboratory Center, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China

Abstract

It is the first time that mRNA-LNPs have been used to express anti-HBsAg antibodies (G12-scFv, G12-scFv-Fc, and G12-IgG). G12-scFv-Fc- and G12-IgG-encoding mRNA-LNPs exerted a sustained effect on HBsAg serum clearance in the adeno-associated virus (AAV)/HBV mouse model with persistent HBsAg expression.

Funder

the National Key R&D Program of China

National Natural Science Foundation of China

Shanghai Municipal Education Commission

Shanghai Municipal Health Commission

Science and Technology Commission of Shanghai Municipality

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

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