Draft Genome Sequences of Seven Strains of Shiga Toxin-Producing Escherichia coli O111 with Variation in Their Sensitivity to Novobiocin

Author:

Rotundo Luca1,Boccia Federica2,Fratamico Pina M.1,Xu Aixia1,Sommers Christopher H.1,Liu Yanhong1,Bono James L.3,Pepe Tiziana2

Affiliation:

1. USDA, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania, USA

2. Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy

3. USDA, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, Nebraska, USA

Abstract

Inclusion of novobiocin as a selective agent for enrichment media and selective agars inhibits the growth of some Shiga toxin-producing Escherichia coli (STEC) strains, particularly non-O157 STEC, which can yield false-negative detection results. Here, we report the draft genomic sequences of seven STEC O111 isolates with different sensitivities to novobiocin.

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Reference18 articles.

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3. International Organization for Standardization. 2001. Microbiology of food and animal feeding stuffs—horizontal method for the detection of Escherichia coli O157. ISO16654:2001. International Organization for Standardization, Geneva, Switzerland. https://www.iso.org/standard/29821.html.

4. International Organization for Standardization. 2012. Microbiology of food and animal feed—real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens—horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups. ISO/TS13136:2012. International Organization for Standardization, Geneva, Switzerland. https://www.iso.org/standard/53328.html.

5. US Food and Drug Administration. Bacteriological analytical manual (BAM), 8th ed (Revised, Chapter 4A). US Department of Agriculture, Washington, DC. http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm070080.htm.

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