Cell Culture-Taqman PCR Assay for Evaluation of Cryptosporidium parvum Disinfection

Abstract

ABSTRACT Cryptosporidium parvum represents a challenge to the water industry and a threat to public health. In this study, we developed a cell culture-quantitative PCR assay to evaluate the inactivation of C. parvum with disinfectants. The assay was validated by using a range of disinfectants in common use in the water industry, including low-pressure UV light (LP-UV), ozone, mixed oxidants (MIOX), and chlorine. The assay was demonstrated to be reliable and sensitive, with a lower detection limit of a single infectious oocyst. Effective oocyst inactivation was achieved (>2 log 10 units) with LP-UV (20 mJ/cm 2 ) or 2 mg of ozone/liter (for 10 min). MIOX and chlorine treatments of oocysts resulted in minimal effective disinfection, with <0.1 log 10 unit being inactivated. These results demonstrate the inability of MIOX to inactivate Cryptosporidium . The assay is a valuable tool for the evaluation of disinfection systems for drinking water and recycled water.