Next-Generation Sequencing Analysis of the Within-Host Genetic Diversity of Influenza A(H1N1)pdm09 Viruses in the Upper and Lower Respiratory Tracts of Patients with Severe Influenza-Reference-Cited by-同舟云学术

Next-Generation Sequencing Analysis of the Within-Host Genetic Diversity of Influenza A(H1N1)pdm09 Viruses in the Upper and Lower Respiratory Tracts of Patients with Severe Influenza

Published:2021-02-24 Issue:1 Volume:6 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Takayama Ikuyo¹^ORCID,Nguyen Binh Gia²,Dao Co Xuan²,Pham Thach The²,Dang Tuan Quoc²,Truong Phuong Thai²,Do Thanh Van²,Pham Thuy Thi Phuong³,Fujisaki Seiichiro¹,Odagiri Takato¹,Hasegawa Hideki¹,Nakajima Noriko⁴

Affiliation:

1. Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan

2. Bach Mai Hospital, Hanoi, Vietnam

3. NCGM-Bach Mai Hospital Medical Collaboration Center, Hanoi, Vietnam

4. Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan

Abstract

The D222G/N substitution in the hemagglutinin (HA) protein of influenza A(H1N1)pdm09 virus has been reported to be associated with disease severity and mortality in numerous previous studies. In the present study, 75% of lower respiratory samples contained heterogeneous influenza populations that carried different amino acids at position 222 of the HA protein, whereas all upper respiratory samples only contained the wild-type 222D.

Funder

Japan Agency for Medical Research and Development

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSphere.01043-20

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