Escherichia coli Physiology in Luria-Bertani Broth

Author:

Sezonov Guennadi1,Joseleau-Petit Danièle1,D'Ari Richard1

Affiliation:

1. Institut Jacques Monod, CNRS, Université Paris 6, Université Paris 7, F-75251 Paris Cedex 05, France

Abstract

ABSTRACT Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD 600 ) of 7. Surprisingly, however, steady-state growth ceases at an OD 600 of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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