Affiliation:
1. Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030,1 and
2. Center for Pharmaceutical Biotechnology, The University of Illinois, Chicago, Illinois 606072
Abstract
ABSTRACT
rRNA plays a central role in protein synthesis and is intimately involved in the initiation, elongation, and termination stages of translation. However, the mode of its participation in these reactions, particularly as to the decoding of genetic information, remains elusive. In this paper, we describe a new approach that allowed us to identify an rRNA segment whose function is likely to be related to translation termination. By screening an expression library of random rRNA fragments, we identified a fragment of the
Escherichia coli
23S rRNA (nucleotides 74 to 136) whose expression caused readthrough of UGA nonsense mutations in certain codon contexts in vivo. The antisense RNA fragment produced a similar effect, but in neither case was readthrough of UAA or UAG observed. Since termination at UGA in
E. coli
specifically requires release factor 2 (RF2), our data suggest that the fragments interfere with RF2-dependent termination.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
15 articles.
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