Improvement of avian leukosis virus (ALV)-based retrovirus vectors by using different cis-acting sequences from ALVs-Reference-Cited by-同舟云学术

Improvement of avian leukosis virus (ALV)-based retrovirus vectors by using different cis-acting sequences from ALVs

Published:1991-06 Issue:6 Volume:65 Page:3388-3394
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Cosset F L¹,Legras C¹,Thomas J L¹,Molina R M¹,Chebloune Y¹,Faure C¹,Nigon V M¹,Verdier G¹

Affiliation:

1. Institut National de la Recherche Agronomique, Université Claude Bernard Lyon-I, Villeurbanne, France.

Abstract

Production and expression of double-expression vectors which transduce both Neo(r) and lacZ genes and are based on the structure of avian leukosis virus were enhanced by using cis-acting sequences (long terminal repeats and noncoding sequences) from Rous-associated virus-1 and Rous-associated virus-2 rather than those of avian erythroblastosis virus previously used in our constructs. Polyclonal producer cells obtained after transfection of these vectors into the Isolde packaging cell line gave rise to titers as high as 3 x 10(5) lacZ CFU/ml, whereas it was possible to isolate clones of producer cells giving rise to titers of more than 10(6) resistance focus-forming units per ml.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.65.6.3388-3394.1991

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