Evaluation of Three Molecular Assays for Rapid Identification of Methicillin-Resistant Staphylococcus aureus-Reference-Cited by-同舟云学术

Evaluation of Three Molecular Assays for Rapid Identification of Methicillin-Resistant Staphylococcus aureus

Published:2007-06 Issue:6 Volume:45 Page:2011-2013
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Francois Patrice¹,Bento Manuela¹,Renzi Gesuele²,Harbarth Stephan³,Pittet Didier³,Schrenzel Jacques¹²

Affiliation:

1. Department of Internal Medicine, Service of Infectious Diseases/Genomic Research Laboratory

2. Clinical Microbiology Laboratory

3. Infection Control Program, University of Geneva Hospitals, Rue Micheli-du-Crest 24, CH-1211 Geneva 14, Switzerland

Abstract

ABSTRACT One home-developed assay and two commercial assays for the rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) were compared by use of a collection of clinical isolates displaying highly diverse genetic backgrounds. Our results suggest that users of orfX -staphylococcal cassette chromosome mec -based assays should repeatedly monitor the local epidemiology to minimize the risks of detection bias and the omission of emerging MRSA clones.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00232-07

Reference23 articles.

1. Does Nasal Cocolonization by Methicillin-Resistant Coagulase-Negative Staphylococci and Methicillin-Susceptible Staphylococcus aureus Strains Occur Frequently Enough To Represent a Risk of False-Positive Methicillin-Resistant S. aureus Determinations by Molecular Methods?

2. Berglund, C., P. Molling, L. Sjoberg, and B. Soderquist. 2005. Predominance of staphylococcal cassette chromosome mec (SCCmec) type IV among methicillin-resistant Staphylococcus aureus (MRSA) in a Swedish county and presence of unknown SCCmec types with Panton-Valentine leukocidin genes. Clin. Microbiol. Infect.11:447-456.

3. Concurrent Analysis of Nose and Groin Swab Specimens by the IDI-MRSA PCR Assay Is Comparable to Analysis by Individual-Specimen PCR and Routine Culture Assays for Detection of Colonization by Methicillin-Resistant Staphylococcus aureus

4. Chaix, C., I. Durand-Zaleski, C. Alberti, and C. Brun-Buisson. 1999. Control of endemic methicillin-resistant Staphylococcus aureus: a cost-benefit analysis in an intensive care unit. JAMA282:1745-1751.

5. Performance standards for antimicrobial susceptibility testing: 15th informational supplement M100-S15. 2005

Cited by 71 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Rapid Point-of-Care PCR Testing of Drug-Resistant Strains on Endotracheal Aspirate Samples: A Repurposed Effective Tool in the Stepwise Approach of Healthcare-Acquired Pneumonia—A Pilot Study;International Journal of Molecular Sciences;2023-08-29

2. A Novel Strategy to Identify Endolysins with Lytic Activity against Methicillin-Resistant Staphylococcus aureus;International Journal of Molecular Sciences;2023-03-17

3. Antimicrobial Management of Complicated Skin and Soft Tissue Infections in an Era of Emerging Multi-Drug Resistance: Perspectives from 5 Gulf Countries;Journal of Acute Care Surgery;2021-11-30

4. Impact of Total Laboratory Automation on Turnaround Times for Urine Cultures and Screening Specimens for MRSA, ESBL, and VRE Carriage: Retrospective Comparison With Manual Workflow;Frontiers in Cellular and Infection Microbiology;2020-10-28

5. Rapid Detection of mecA and femA Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance;Frontiers in Microbiology;2020-07-09