Genetic Organization of the Downstream Region of the mecA Element in Methicillin-Resistant Staphylococcus aureus Isolates Carrying Different Polymorphisms of This Region-Reference-Cited by-同舟云学术

Genetic Organization of the Downstream Region of the mecA Element in Methicillin-Resistant Staphylococcus aureus Isolates Carrying Different Polymorphisms of This Region

Published:2000-07 Issue:7 Volume:44 Page:1906-1910
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Oliveira Duarte C.¹²,Wu Shang W.²,de Lencastre Hermínia¹²

Affiliation:

1. Molecular Genetics Unit, Instituto de Tecnologia Quı́mica e Biológica da Universidade Nova de Lisboa, Oeiras, Portugal,1 and

2. Laboratory of Microbiology, The Rockefeller University, New York, New York 100212

Abstract

ABSTRACT We describe here the genetic organization of the mec element downstream of the mecA gene in 34 different methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates carrying 13 of the most frequent polymorphisms of mecA and representing the major epidemic clones of MRSA. All polymorphisms carried three common genetic elements: the hypervariable region, a copy of IS 431 , and a unique 2-kb sequence (downstream constant segment, or dcs ) for which no homologous sequences are found in data banks. Polymorphisms of the downstream region were shown to be caused by the presence of linearized plasmids flanked by insertion sequences (pUB110, pT181, and pI258) and the autonomous insertion sequence IS 256 .

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.44.7.1906-1910.2000

Reference29 articles.

1. Origin and evolution of DNA associated with resistance to methicillin in staphylococci.;Archer G. L.;Trends Microbiol.,1994

2. Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. Short protocols in molecular biology. 1992 Green Publishing Associates and John Wiley & Sons New York N.Y

3. Spread of a methicillin-resistant and multiresistant epidemic clone of Staphylococcus aureus in Argentina.;Corso A.;Microb. Drug Resist.,1998

4. Archaic clones of methicillin-resistant Staphylococcus aureus (MRSA): molecular and microbiological properties of isolates from the 1960s in Denmark.;de Lencastre H.;Microb. Drug Resist.,2000

5. Methicillin-resistant Staphylococcus aureus disease in a Portuguese hospital: characterization of clonal types by a combination of DNA typing methods.;de Lencastre H.;Eur. J. Clin. Microbiol. Infect. Dis.,1994

Cited by 56 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A Review on Five and Six-Membered Heterocyclic Compounds Targeting the Penicillin-Binding Protein 2 (PBP2A) of Methicillin-Resistant Staphylococcus aureus (MRSA);Molecules;2023-10-10

2. Nucleic acid amplification-based microfluidic approaches for antimicrobial susceptibility testing;The Analyst;2021

3. Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA;Methods in Molecular Biology;2019-09-16

4. Molecular Typing of ST239-MRSA-III From Diverse Geographic Locations and the Evolution of the SCCmec III Element During Its Intercontinental Spread;Frontiers in Microbiology;2018-07-06

5. Variation in MRSA identification results from different generations of Xpert MRSA real-time PCR testing kits from nasal swabs;Journal of Infection and Public Health;2017-11