Affiliation:
1. Departments of Biology1 and
2. Chemistry,2 The University of Konstanz, D-78457 Konstanz, Germany
Abstract
ABSTRACT
Enrichment cultures with enantiomeric 2-(4-sulfophenyl)butyrate (SPB) as the sole added source(s) of carbon and energy for growth yielded a pure culture of a degradative bacterium, which was identified as
Delftia acidovorans
SPB1. The organism utilized the enantiomers sequentially.
R
-SPB was utilized first (specific growth rate [μ] = 0.28 h
−1
), with transient excretion of an unknown intermediate, which was identified as 4-sulfocatechol (4SC). Utilization of
S
-SPB was slower (μ = 0.016 h
−1
) and was initiated only after the first enantiomer was exhausted. Suspensions of cells grown in
S
-SPB excreted 4SC, so metabolism of the two enantiomers converged at 4SC. The latter was degraded by
ortho
cleavage via 3-sulfo-
cis
,
cis
-muconate. Strain SPB1 grew with 4SC and with 1-(4-sulfophenyl)octane (referred to herein as model LAS) but not with commercial linear alkylbenzenesulfonate (LAS) surfactant, which is subterminally substituted but nontoxic. It would appear that metabolism of the model LAS does not represent metabolism of commercial LAS.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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