Nontoxigenic strains of Clostridium difficile lack the genes for both toxin A and toxin B-Reference-Cited by-同舟云学术

Nontoxigenic strains of Clostridium difficile lack the genes for both toxin A and toxin B

Published:1991-11 Issue:11 Volume:29 Page:2666-2667
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fluit A C¹,Wolfhagen M J¹,Verdonk G P¹,Jansze M¹,Torensma R¹,Verhoef J¹

Affiliation:

1. Eijkman Winkler Laboratory for Medical Microbiology, University Hospital Utrecht, The Netherlands.

Abstract

A total of 39 toxigenic and 20 nontoxigenic strains of Clostridium difficile were tested for the presence of either toxin A or toxin B by the polymerase chain reaction (PCR). All toxigenic strains produced cytotoxin as assayed by using highly sensitive fetal lung fibroblasts and were positive for toxin A as well as toxin B in the PCR assay. All nontoxigenic strains failed to produce toxin and were negative in the PCR assay. This study shows that nontoxigenic strains of Clostridium difficile lack the toxin A as well as the toxin B gene.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.29.11.2666-2667.1991

Reference11 articles.

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