Multiplex PCR Assay for Rapid and Accurate Capsular Typing of Group B Streptococci-Reference-Cited by-同舟云学术

Multiplex PCR Assay for Rapid and Accurate Capsular Typing of Group B Streptococci

Published:2007-06 Issue:6 Volume:45 Page:1985-1988
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Poyart Claire¹²³,Tazi Asmaa¹²³,Réglier-Poupet Hélène¹²³,Billoët Annick¹,Tavares Nicole¹,Raymond Josette¹,Trieu-Cuot Patrick²⁴

Affiliation:

1. Service de Bactériologie

2. Centre National de Référence des Streptocoques, Groupe Hospitalier Cochin-Saint Vincent de Paul, Assistance Publique-Hôpitaux de Paris, 27 rue du Faubourg Saint Jacques, 75679 Paris, France

3. Institut Cochin-INSERM U567-UMR CNRS 810, Faculté de Médecine Université Descartes, 22 rue Méchain, 75014 Paris, France

4. Unité de Biologie des Bactéries Pathogènes à Gram-Positif-URA CNRS 2172, Institut Pasteur, 75724 Paris, France

Abstract

ABSTRACT We developed a simple, specific, and sensitive two-multiplex-PCR assay that enabled the detection of all known group B streptococcal (GBS) capsular polysaccharides. This test is well adapted for GBS capsular polysaccharide typing in large-scale epidemiological studies.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00159-07

Reference17 articles.

1. DNA Macrorestriction Analysis of Nontypeable Group B Streptococcal Isolates: Clonal Evolution of Nontypeable and Type V Isolates

2. Comparison of DNA Dot Blot Hybridization and Lancefield Capillary Precipitin Methods for Group B Streptococcal Capsular Typing

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4. Isolation, chemical composition, and molecular size of extracellular type II and type Ia polysaccharides of group B streptococci

5. Glaser, P., C. Rusniok, C. Buchrieser, F. Chevalier, L. Frangeul, T. Msadek, M. Zouine, E. Couve, L. Lalioui, C. Poyart, P. Trieu-Cuot, and F. Kunst. 2002. Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease. Mol. Microbiol.45:1499-1513.

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