The Cys607→Tyr Change in the UL97 Phosphotransferase Confers Ganciclovir Resistance to Two Human Cytomegalovirus Strains Recovered from Two Immunocompromised Patients-Reference-Cited by-同舟云学术

The Cys607→Tyr Change in the UL97 Phosphotransferase Confers Ganciclovir Resistance to Two Human Cytomegalovirus Strains Recovered from Two Immunocompromised Patients

Published:1998-02 Issue:2 Volume:42 Page:444-446
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Baldanti Fausto¹,Underwood Mark R.²,Talarico Christine L.²,Simoncini Lavinia¹,Sarasini Antonella¹,Biron Karen K.²,Gerna Giuseppe¹

Affiliation:

1. Servizio di Virologia, IRCCS Policlinico S. Matteo, 27100 Pavia, Italy,1 and

2. Department of Virology, Glaxo Wellcome Inc., Research Triangle Park, North Carolina 277092

Abstract

ABSTRACT Two ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) strains recovered from an AIDS patient (strain VR4990) and a heart transplant recipient (strain VR5474) showed a Cys607→Tyr change in the UL97-encoded phosphotransferase. No amino acid substitutions were observed in the viral DNA polymerase. Marker transfer experiments showed marked reduction in GCV phosphorylation and drug susceptibility of the recombinant HCMV strain VR4990rec2-1-1. These results further extend the region of the carboxy-terminal domain of the UL97 phosphotransferase involved in GCV substrate recognition.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.42.2.444

Reference25 articles.

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