Efficient Reactivation of Latent Herpes Simplex Virus from Mouse Central Nervous System Tissues

Author:

Chen Shih-Heng1,Yao Hui-Wen1,Huang Wen-Yen1,Hsu Kuei-Sen12,Lei Huan-Yao13,Shiau Ai-Li13,Chen Shun-Hua13

Affiliation:

1. Institute of Basic Medical Sciences

2. Department of Pharmacology

3. Department of Microbiology and Immunology, Medical College, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China

Abstract

ABSTRACT For decades, numerous ex vivo studies have documented that latent herpes simplex virus (HSV) reactivates efficiently from ganglia, but rarely from the central nervous systems (CNS), of mice when assayed by mincing tissues before explant culture, despite the presence of viral genomes in both sites. Here we show that 88% of mouse brain stems reactivated latent virus when they were dissociated into cell suspensions before ex vivo explant culture. The efficient reactivation of HSV from the mouse CNS was demonstrated with more than one viral strain, viral serotype, and mouse strain, further indicating that the CNS can be an authentic latency site for HSV with the potential to cause recurrent disease.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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