Affiliation:
1. Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
Abstract
ABSTRACT
Bacillus cereus
569 (ATCC 10876) germinates in response to inosine or to
l
-alanine, but the most rapid germination response is elicited by a combination of these germinants. Mutants defective in their germination response to either inosine or to
l
-alanine were isolated after Tn 917 -LTV1 mutagenesis and enrichment procedures; one class of mutant could not germinate in response to inosine as a sole germinant but still germinated in response to
l
-alanine, although at a reduced rate; another mutant germinated normally in response to inosine but was slowed in its germination response to
l
-alanine. These mutants demonstrated that at least two signal response pathways are involved in the triggering of germination. Stimulation of germination in
l
-alanine by limiting concentrations of inosine and stimulation of germination in inosine by low concentrations of
l
-alanine were still detectable in these mutants, suggesting that such stimulation is not dependent on complete functionality of both these germination loci. Two transposon insertions that affected inosine germination were found to be located 2.2 kb apart on the chromosome. This region was cloned and sequenced, revealing an operon of three open reading frames homologous to those in the
gerA
and related operons of
Bacillus subtilis
. The individual genes of this
gerI
operon have been named
gerIA
,
gerIB
, and
gerIC
. The GerIA protein is predicted to possess an unusually long, charged, N-terminal domain containing nine tandem copies of a 13-amino-acid glutamine- and serine-rich sequence.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
119 articles.
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