Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example
Author:
Affiliation:
1. INRA, University of Burgundy, Soil and Environmental Microbiology, CMSE, 17 Rue Sully, B.P. 86510, 21065 Dijon Cedex, France
Abstract
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Link
https://journals.asm.org/doi/pdf/10.1128/AEM.02403-07
Reference16 articles.
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2. Baker, G. C., J. J. Smith, and D. A. Cowan. 2003. Review and re-analysis of domain-specific 16S primers. J. Microbiol. Methods55:541-555.
3. Day, P. J., D. Bergstrom, R. P. Hammer, and F. Barany. 1999. Nucleotide analogs facilitate base conversion with 3′ mismatch primers. Nucleic Acids Res.1999:1810-1818.
4. Real-Time PCR Assay for Detection and Genotype Differentiation of Giardia lamblia in Stool Specimens
5. Head, I. M., J. R. Saunders, and R. W. Pickup. 1998. Microbial evolution, diversity and ecology: a decade of ribosomal RNA analysis of uncultivated microorganisms. Microb. Ecol.35:1-21.
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