New Plasmid System To Select for Saccharomyces cerevisiae Purine-Cytosine Permease Affinity Mutants-Reference-Cited by-同舟云学术

New Plasmid System To Select for Saccharomyces cerevisiae Purine-Cytosine Permease Affinity Mutants

Published:2001-07-15 Issue:14 Volume:183 Page:4386-4388
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Wagner Renaud¹,Straub Marie-Laure¹,Souciet Jean-Luc¹,Potier Serge¹,de Montigny Jacky¹

Affiliation:

1. Laboratoire de Microbiologie et de Génétique, ESA 7010 Université Louis Pasteur/CNRS, Institut de Botanique, F-67083 Strasbourg Cedex, France

Abstract

ABSTRACT The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.183.14.4386-4388.2001

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