Affiliation:
1. Department of Biochemistry, University of Georgia, Athens, Georgia 30602
Abstract
The formation of the nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase in
Clostridium thermoaceticum
is stimulated by the presence of molybdate and selenite in the growth medium. The highest formate dehydrogenase activity was obtained with 2.5 × 10
−4
M Na
2
MoO
4
and 5 × 10
−5
Na
2
SeO
3
. Tungstate but not vanadate could replace molybdate and stimulate the formation of formate dehydrogenase. Tungstate stimulated activity more than molybdate, and in combination with molybdate the stimulation of formation of formate dehydrogenase was additive. Formate dehydrogenase was isolated from cells grown in the presence of Na
2
75
SeO
2
, and a correlation was observed between bound
75
Se and enzyme activity.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference21 articles.
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