Affiliation:
1. National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Abstract
The serodiagnosis of human psittacosis was considerably improved by a microimmunofluorescence (MIF) assay that uses selected strains of Chlamydia psittaci, C. pneumoniae, and C. trachomatis as antigens. The 78 patients examined in the study were clinically diagnosed as having psittacosis on the basis of compatible clinical symptoms following exposure to sick birds. The conventional complement fixation (CF) test identified 36 patients, or 46% (36 of 78) of the total, as positive. Antibody responses to C. psittaci were demonstrated by the MIF test in all 36 CF-positive patients. The MIF test also detected antibody responses to C. psittaci in 12 patients (15% of the total) whose sera were negative or anticomplementary in the CF test. Seven patients, or 9% (7 of 78) of the total, were identified by the MIF test as having C. pneumoniae infections. About 30% of the study patients (23 of 78) showed no serologic evidence of either C. psittaci or C. pneumoniae infection by both the CF and the MIF tests. Four distinctive serologic reaction patterns were observed in the study patients. Recognition of these reaction patterns and judicious corroboration of serologic responses to the chlamydial species by the MIF test with epidemiologic and clinical information will increase the efficiency and accuracy of serodiagnosis for human psittacosis.
Publisher
American Society for Microbiology
Reference15 articles.
1. Serotyping of Chlamydia psittaci isolates using serovar-specific monoclonal antibodies with the microimmunofluorescence test;Andersen A. A.;J. Clin. Microbiol.,1991
2. A clinical and epidemiological study of "ornithosis;Fryden A.;Scand. J. Infect. Dis.,1989
3. Immunochemical diversity of the major outer membrane protein of avian and mammalian Chlamydia psittaci;Fukushi H.;J. Clin. Microbiol.,1988
4. Psittacosis: a diagnostic challenge;Kuritsky J. N.;J. Occup. Med.,1984
5. Palmer D. F. 1981. A guide to the performance of the standardized diagnostic complement fixation method and adaptation to micro-test. Centers for Disease Control Atlanta.
Cited by
40 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献