Genetic Determinants of Rifampicin Resistance of Mycobacterium tuberculosis not Included in the Russian Molecular Genetic Test Systems
-
Published:2023-07-04
Issue:3
Volume:101
Page:69-77
-
ISSN:2542-1506
-
Container-title:Tuberculosis and Lung Diseases
-
language:
-
Short-container-title:Tuberk. bolezni lëgk.
Author:
Mazurina E. A.1, Umpeleva T. V.1, Golubeva L. A.1, Lavrenchuk L. S.1, Vakhrusheva D. V.1, Vasilyeva I. A.2
Affiliation:
1. Ural Phthisiopulmonology Research Institute – a Branch of National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health 2. National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Abstract
The objective: to study the genetic determinants of rifampicin resistance of Mycobacterium tuberculosis by sequencing the RRDR rpoB gene.Subjects and Methods. The study included 651 M. tuberculosis isolates that were tested for drug suceptibility (DST) by genotypic methods (gDST) TB-TEST (BIOCHIP-IMB, Russia), and Amplitube-MDR-RV (NPK Sintol, Russia) and phenotypic methods (phDST): the absolute concentration method, BACTEC MGIT 960 system, and Sensititre Myco TB kit. Sanger RRDR sequencing of the rpoB gene was performed for 20 isolates with discrepancies in the results of phDST and gDST.Results. As a result of sequencing, two variants of mutations in the RRDR region of the rpoB gene were found, which were not detected by TB-TEST and Amblitub MDR-RV test systems, however were strongly associated with rifampicin resistance of Mycobacterium tuberculosis. The most common variant was the insertion of three nucleotides (TTC) encoding the amino acid phenylalanine at position 434 of the codon (1296-1300insTTC).
Publisher
LLC "Medical Knowledge and Technologies"
Reference30 articles.
1. Klinicheskie rekomendatsii Tuberkulez u vzroslykh. [Clinical guidelines on tuberculosis in adults]. 2022, All-Russia Non-Commercial Organization of the Russian Society of Phthisiologists, http://cr.minzdrav.gov.ru/recomend/16 2. Umpeleva T. V., Mazurina E. A., Vakhrusheva D. V., Eremeeva N. I. Comparison of different methods for drug susceptibility testing of Mycobacterium tuberculosis to rifampicin. Tuberculosis and Lung Diseases, 2022, vol. 100, no. 1, pp. 41–48. (In Russ.) https://doi.org/10.21292/2075-1230-2022-100-1-41-48 3. Chernousova L. N., Sevastianova E. V., Larionova E. E., Smirnova T. G., Andreevskaya S. N., Popov S. A., Zhuravlev V. Yu., Puzanov V. A., Maryandyshev A. O., Vakhrusheva D. V., Kravchenko M. A., Safonova S. G., Vasilyeva I. A., Ergeshov A. E. Federalnye klinicheskie rekomendatsii po organizatsii i provedeniyu mikrobiologicheskoy i molekulyarno-geneticheskoy diagnostiki tuberkuleza. [Federal clinical recommendations in organization and implementation of microbiological and molecular-genetic diagnostics of tuberculosis]. Moscow, 2014. 4. Afanas’ev M. V., Ikryannikova L. N., Il’ina E. N., Sidorenko S. V., Kuz’min A. V., Larionova E. E., Smirnova T. G., Chernousova L. N., Kamaev E. Y., Skorniakov S. N., Kinsht V. N., Cherednichenko A. G., Govorun V. M. Molecular characteristics of rifampicin- and isoniazid-resistant Mycobacterium tuberculosis isolates from the Russian Federation // Journal of Antimicrobial Chemotherapy – 2007. – Vol. 59, № 6. – P. 1057–1064. https://doi.org/10.1093/JAC/DKM086 5. Chen X., Wang B., Yang W., Kong F., Li, C., Sun Z., Jelfs P., Gilbert G. L. Rolling circle amplification for direct detection of rpoB gene mutations in Mycobacterium tuberculosis isolates from clinical specimens // Journal of Clinical Microbiology – 2014. – Vol. 52, № 5. – P. 1540–1548. https://doi.org/10.1128/JCM.00065-14/ASSET/60AB97CA-328F-42FF-8CAD-107232BFDE6F/ASSETS/GRAPHIC/ZJM999093342002B.JPEG
|
|