An in vivo and in vitro photopolar array for precision optogenetic stimulation

Abstract

We propose the use of micro-LED arrays as a powerful tool to achieve precise stimulation of neuronal cells in time and space. In particular, we have developed a control system for in vivo implant stimulation and in vitro neuronal stimulation of organisms, through which up to 4096 individual micro-LED light sources can be controlled. The system can be integrated into microscopes and membrane clamp electrophysiology devices for efficient optogenetic monitoring. The final experiments show that different combinations of irradiation of the photopolar array can be used to effectively stimulate neurons transfected with photosensitive proteins by varying the light power.