Affiliation:
1. From the Institute of Experimental Biology and the Department of Anatomy, University of California, Berkeley
Abstract
The solubility of sheep pituitary lactogenic hormone in 0.302 M NaCl at pH 2.02 (solution in HCl) has been determined at room temperature. It showed a constant solubility in the presence of a considerable excess of the solid phase, an indication that the preparation contained but one component.
Beef lactogenic hormone showed a constant solubility in distilled H2O at 7–8°C in the presence of excess of the solid phase.
The salting-out effect of NaCl in acid solution of both beef and sheep hormones has been studied at room temperature. In these studies both preparations behaved as pure substances, but they exhibited differences in solubility, thus indicating a species specificity.
Publisher
Rockefeller University Press
Cited by
21 articles.
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1. Studies on Human Pituitary Growth and Gonadotropic Hormones;Ciba Foundation Symposium - Human Pituitary Hormones (Colloquia on Endocrinology, Vol. 13);2008-05-21
2. Human pituitary growth hormone: Solubility in ammonium sulfate solutions;Archives of Biochemistry and Biophysics;1974-04
3. Occurence of several active components in ovine lactogenic hormone as revealed by zone electrophoresis on starch;Biochimica et Biophysica Acta;1959-06
4. Hormonal synergism in mammary growth;Proceedings of the Royal Society of London. Series B - Biological Sciences;1958-12-17
5. Biochemistry of the Protein Hormones;Annual Review of Biochemistry;1958-06