<i>In vitro</i> cell-based Hyperuricemia-hemotest bioassay for cytokine status evaluation in patients with gouty arthritis

Abstract

Aim– to develop anin vitromethod for assessing the activity of the inflammasome under conditions of hyperuricemic stimulation of inflammatory interleukins. Material and methods.Whole blood cells of donors and patients with hyperuricemia and exacerbation of gouty arthritis diluted with RPMI were culturedin vitroin the presence of different concentrations of uric acid. The production of cytokines in the cell growth media of hematopoietic cells stimulated with uric acid was evaluated using an enzyme-linked immunosorbent assay (ELISA). Results.By simulating the hyperuricemiain vivo, anin vitrocell-based bioassay was developed to stimulate blood cells of individual donors with uric acid. Using the developedin vitroHyperuricemia-hemotest bioassay, quantitative differences were found in the production of inflammatory cytokines by the blood cells of potentially healthy donors and patients with hyperuricemia and gouty arthritis. Conclusion.As a new approach in personalized diagnostics, a hyperuricemic (HU)-hemotest system was developed, which can serve as anin vitrocell model for studying the activation of inflammasome by inflammatory signaling molecules in gouty arthritis.