The Impact of Different rs3917538 Genotypes with the PON-1 Activity, Atherosclerosis Severity in Patients of Coronary Atherosclerosis

Abstract

Background: Coronary atherosclerosis is one of the coronary artery diseases (CAD) responsible for a significant percentage of mortality and morbidity in Iraqi subjects. Atherosclerosis compresses about 17 million death cases; 45% are under the age of 70. The improvement in atherosclerosis disease management paly a critical role in preventing this disease; despite that, new risk factors have been studied, including genetic studies. Many polymorphisms have been associated with coronary atherosclerosis in different ethnic populations. Paraoxonase is an enzyme located on HDL in serum. PON acts as a protective factor or agent against oxidative modification of LDL, suggesting that it could play an essential and influential role in atherosclerotic processes prevention. Objective: To evaluate the rs3917538 genotypes in the PON-1 gene associated with the coronary atherosclerosis risk and the effect of this single nucleotide polymorphism on the serum activity of PON-1 and the severity of coronary atherosclerosis. Methods: A case-control study included sixty patients diagnosed with coronary atherosclerosis and sixty healthy volunteers diagnosed with angiography examination. The study was carried out during the period from February 2019 till June 2020. Serum PON-1 was measured by fluorometric technique, and the genetic assessment was by RT-PCR technique. Results: The activity for PON-1 in patients was significantly lower than controls; the median activity of PON-1 in controls was 197.32 mU/ml, which was higher than that of patients (median =163.46 mU/ml). There was an impact of rs3917538 genotypes on the severity of the disease, P=0.041. The rs3917538 genotypes have no association with the risk of coronary atherosclerosis and the activity of PON-1. Conclusion: The measurement of serum PON-1 activity may help in the diagnosis  of coronary atherosclerosis patients. The rs3917538 genotypes in the PON-1 gene among the patient’s group impact the severity of coronary atherosclerosis.