Author:
Afdal Afdal,Jamsari Jamsari,Yanwirasti Yanwirasti,Rukmono Prambudi
Abstract
Background: Neonatal sepsis is a problem in the field of child health because the incidence is increasing every year and often ends in death. This study aimed to investigate the relationship between TNF-α and BPI gene polymorphisms with neonatal sepsis.
Methods: PCR and sequencing examinations were performed on 60 DNA samples consisting of 30 samples of neonatal sepsis and 30 samples of non-neoptic sepsis. Furthermore, data in the form of DNA mutation tables were statistically processed by univariate, bivariate, and multivariate analysis. It is considered meaningful if p<0.05.
Result: The results showed that BPI rs1341023, rs5743507 and TNF-α rs361525, rs1800629 only BPI rs1341023 and TNF-α rs1800629 were mutated. Of the two genes, only TNF-α rs1800629 had a significant association with neonatal sepsis (p<0.05).
Conclusion: This study proved that the TNF-α rs1800629 mutation was the most important polymorphism in the occurrence of neonatal sepsis (p<0.05).
Keywords: BPI, TNF-α, Neonatal, Polymorphism, Sepsis
Publisher
Scientific Foundation SPIROSKI
Reference13 articles.
1. Fleischmann C, Scherag A, Adhikari NK, Hartog CS, Tsaganos T, Schlattmann P, et al. Assessment of Global Incidence and mortality of hospital-treated sepsis. Current estimates and limitations. Am J Respir Crit Care Med. 2016;193(3):259-72. https://doi.org/10.1164/rccm.201504-0781OC PMid:26414292
2. Kumar G, Kumar N, Taneja A, Kaleekal T, Tarima S, McGinley E, et al. Nationwide trends of severe sepsis in the 21st century (2000-2007). Chest. 2011;140(5):1223-31. https://doi.org/10.1378/chest.11-0352 PMid:21852297
3. World Health Organization. Sepsis. Tersedia Pada; 2018. Available from: https://www.who.int/news-room/fact-sheets/detail/sepsis [Last accessed on 2020 Jan 08].
4. Nupponen I, Turunen R, Nevalainen T, Peuravuori H, Pohjavuori M, Repo H, et al. Extracellular release of bactericidal/permeability-increasing protein in newborn infants. Pediatr Res. 2002;51(6):670-4. https://doi.org/10.1203/00006450-200206000-00002 PMid:12032259
5. Rukmono P. Polimorfisme Asp2999Gly dan Thr399Ile Gen TLR-4 Pada Sepsis Neonatorum serta Hubungannya dengan Kadar TNF-α dan IL-6. Indonesian: Universitas Andalas; 2015.