Abstract
Fifty-four roughages of known voluntary dry-matter intakes (DMI; range 7·8−35·2 g/kg live weight per d) were examinedin vitroin a gas production test. Samples (200 mg) of roughage and roughage neutral-detergent fibre (NDF) respectively were incubated in a mixed suspension of rumen contents for 96 h and the gas volumes recorded after 4,6,8,12,24,30,36,48,54,60 and 96 h. The kinetics of gas production were derived from the volume recordings described by the exponential equation Y=A+B(l—e-ct) where A is the intercept and ideally reflects the fermentation of the soluble and readily available fraction of the feed,Bdescribes the fermentation of the insoluble (but with time fermentable) fraction andcthe fractional rate at whichBis fermented per h;A+Bdescribes total fermentation.In vitrotrue dry matter (TD) and NDF degradabilities (NDF-D) after 24 h incubation were also determined. Of the variation in DMI, 75% was accounted for by thein vitrogas production parametersA, Bandcin stepwise multiple regressions; 82% of the variation in DMI was explained by the parameters (ANDF+BNDF) and cNDFas obtained from the incubation of roughage NDF. The rate constants (c) were less important than parameters related to the extent of gas production, accounting for only 6·5 (whole roughage) and 4·1% (NDF) of the variation in DMI. There was no statistical advantage in the use of the exponential model describing extent and rate of fermentation over some of the simple gas volume measurements: 75% of the variation in DMI was accounted for by in vitro gas production of whole roughage after 8 h of incubation. On average gas production from NDF measured from 24–96 h accounted for 81% of the variation in DMI. A combination of gas volume measurements after a short period of incubation (4–8 h) with a concomitant determination of NDF-D after many hours (≥24 h) can render NDF preparations and long incubation times redundant. A method is suggested to obtain two results for DMI prediction in one single incubation. Of the variation in DMI 80% was accounted for by the incubation of 500 mg whole roughage when incubation was terminated after 24 h and the residual undegraded substrate quantified.
Publisher
Cambridge University Press (CUP)
Subject
Nutrition and Dietetics,Medicine (miscellaneous)
Cited by
130 articles.
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