Microbial protein and peptide metabolism in rumen fluid from faunated and ciliate-free sheep

Abstract

1. Protease and deaminase activities and the metabolism of peptides were measured in rumen fluid from ciliate- free sheep and from sheep with a limited population of small entodinia. The same measurements were repeated following inoculation of the latter group with a more typical mixed ciliate population.2. Protease and dialanine uptake activities of mixed rumen micro-organisms were not significantly influenced by protozoa. Trialanine uptake, leucine aminopeptidase (EC 3.4.11. 1), deaminase and trypsin-like protease activities were 70, 107,73 and 91 % higher with the limited population, and 72,58,64 and 55 % higher when mixed protozoa were present, indicating a major role for the protozoa in these activities.