Peculiarities of TRP channels expression and cytokine profile of sputum in patients with chronic obstructive pulmonary disease and progressive bronchial obstruction

Author:

Naumov D. E.1,Sugaylo I. Yu.1,Gassan D. A.1,Kotova O. O.1,Gorchakova Ya. G.1,Sheludko E. G.1

Affiliation:

1. Far Eastern Scientific Center of Physiology and Pathology of Respiration

Abstract

Introduction. Chronic obstructive pulmonary disease (COPD) is a pathology accompanied by a pro­gressive and irreversible deterioration in airway patency. It is known that macrophages of the respiratory tract are actively involved in the reorganization of the extracellular matrix leading to the development of bronchial remodeling.Aim. To assess the relationship between the progression rate of bronchial obstruction in COPD, the expression of TRP channels on alveolar macrophages, and the levels of inflammatory markers in the respiratory tract.Materials and methods. The study enrolled 37 patients with COPD, including 23 people with a FEV1 deterioration >50 ml/year and 14 with FEV1 decline <50 ml/year. The expression of TRPV1, TRPV4, TRPA1, TRPM8 channels was determined on alveolar macrophages by flow cytometry. Analysis of cytokines was performed in sputum supernatant by multiplex assay on a flow cytometer.Re­sults. It was found that in patients with progressive bronchial obstruction TRPV4 expression was significantly increased: 14.2 (10.8; 23.4)% vs. 8.6 (3.6; 15.4)% (p=0.03). In addition, in the general group of patients a highly significant inverse correlation was found between TRPV4 expression and the dynamics of FEV1 (p=-0.52, p<0.001). Patients with a decrease in FEV1 >50 ml/year were characterized by significantly elevated levels of IL-2, IL-4, IL-17A, IL-10, IL-12p70, CXCL10 and MCP-1. Additionally, we found that concentrations of several cytokines were directly correlated with TRPV4 expres­sion on macrophages: IL-4 (p=0.51, p=0.001), CXCL10 (p=0.59, p<0.001), MCP-1 (p=0.56, p<0.001), TGF-ei (p=0.42, p=0.009), IFN-y (p=0.37, p=0.02).Conclusion. TRPV4 channels expressed on alveolar macrophages are involved in the inflammatory process and airway remodeling in COPD, which is manifested by their relationships with the level of certain cytokines production, as well as the rate of the progression of bronchial obstruction.

Publisher

Far Eastern Scientific Center Of Physiology and Pathology of Respiration

Subject

General Medicine

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3