1. Antigen-specific antibodies of the IgG, IgA, and IgM isotypes and IgG subclasses in serum, SF, or both, were measured by enzyme linked immunosorbent assay (ELISA) using alkaline phosphatase-conjugated mouse anti-human IG1, IgG2, IgG4, IgG, IgA, and IgM antibodies, at 1/500 dilution. The reaction was stopped after 30 minutes and the amount of bound enzyme assayed; absorbance values were read at 405 nm in an ELISA reader (Metertech, 1960). Absorbance was calculated as the mean of triplicate well readings. In each experiment, 65 kDa antigen-specific monoclonal antibody was used as positive control. The optic density (OD) ratio was calculated as OD of the test samples divided by OD of a standard normal serum used in each plate. The control solution (phosphate buffered saline) never gave a value greater than 0-01. The standard deviations of the triplicate wells were all less than 10%;KDA, H.S.P.
2. Studies of polyarthritis and other lesions induced in rats by injection of mycobacteria antigen;M, Pearson C.; D, Wood F.;Arthritis Rheum,1964
3. Arthritis induced by a T lymphocyte clone that responds to Mycobacterium tuberculosis and to cartilage proteoglycan;W, Van Eden; J, Holoshitz; Z, Nevo; A, Frenkel; A, Klajkan; R, Cohen I.;IProc Natl Acad Sci USA,1985
4. Cloning of the mycobacterial epitope recognized by T lymphocyte in adjuvant arthritis;W, Van Eden; R, Thole J.E.; R, Van Der Zee;Nature,1981
5. Synovial fluid T cell reactivity against 65 KD heat shock protein of mycobacterium in early chronic arthritis;M, Res P.C.; G, Schaar C.; C, Breedveld F.;Lancet,1988