The preparation of59Fe-labelled transferrin for ferrokinetic studies

Author:

Cavill I.

Abstract

Serum labelled with59Fe for ferrokinetic studies should contain only [59Fe] transferrin. [59Fe] ferric citrate was incubated at 37°C for up to 24 hours with sera having an excess latent iron-binding capacity. The results showed that unbound [59Fe] ferric citrate may be present for several hours. An anion exchange resin (IRA 400 Cl) column removed all the [59Fe] ferric citrate and left only [59Fe] transferrin in the eluate. It is suggested that all59Fe-labelled preparations for ferrokinetic studies should be passed through a resin column before injection.

Publisher

BMJ

Reference9 articles.

1. The specific binding of iron (111) and copper (11) to transferrin and conalbumin, Biochim;Aasa, R.; Malmstrom, B.G.; Saltman, P.; Vanngard, T.;biophys. Acta (Amst.),1963

2. Citrate-mediated exchange of;P., Aisen; Leibman, A.,1968

3. The kinetics and mechanism of iron (III) exchange between chelates and transferrin. II. The presentation and removal with ethylenediaminetetraacetate;Bates, G.W.; Billups, C.; Saltman, P.;J. biol. Chenm,1967

4. Bearn, A. G., and Parker, W. C. (1966). In Glycoproteins: Their

5. Skin clearance of iron in normal and iron deficient subjects. Brit;Composition, Structure;J. Derni,1970

Cited by 81 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.7亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2025 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3