Affiliation:
1. St. Petersburg State University of Veterinary Medicine
Abstract
Danio rerio is a popular model organism used in various fields of scientific research, including aquaculture. The use of Danio rerio in toxicological studies of drugs promising for use in aquaculture allows reducing the cost of livestock of commercial fish for the needs of experiments. The aim of the study was to evaluate the effect of an organomineral chelate complex on the reproductive function of females and the survival of Danio rerio eggs. An organomineral chelate complex was studied (JUPITER, Russia). The active basis of a biologically active additive (hereinafter dietary supplements): a complex of ethylenediaminediantaric acid and lysine with trace elements (Fe, Mn, Cu, Zn, Co, Se, I). The fish of the experimental groups were given an additive for 30 days, the data were compared with the control. The drug was given together with the feed once a day, in 3 different concentrations (0.25 g / l, 0.125 g / l, 0.0625 g / l). The weight of females at the beginning and end of the experiment, the total number of eggs (from each female for spawning), the number of eggs per 1 g, the survival rate of eggs, the diameter of eggs and the length of larvae 5 dfp (five days after fertilization) were estimated. To obtain caviar, the parent pairs were planted in spawning jigs and left for 12 hours. The effectiveness of the organomineral chelate complex was observed when using it at a dosage of 0.25 g/l. The use of the complex for 30 days led to an increase in the weight of females and the weight of eggs, as well as the number of eggs per spawning. It is also important to note that the use of the drug did not have a significant effect on the survival of the eggs, the diameter of the eggs and the length of the larva. The enhancement of reproductive function and the increase in the size of Danio rerio females can be explained by the influence of the components of the organomineral chelate complex on the course of biochemical reactions in the body of fish.
Publisher
Saint-Petersburg State University of Veterinary Medicine
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