Morphofunctional changes in the reproductive organs of females against the background of the use of a phytopreparation (experimental study)

Abstract

The  paper  presents  the  results  of  the assessment of morphological changes in the uterus  and  ovaries  of  laboratory  animals against the background of the introduction of therapeutic doses of a multifunctional phytopreparation, which includes alcohol extracts from  plants:  white  hellebore  (Veratrum  album),  dioecious  nettle  (Urtica  dioica),  yarrow  (Millefolii  herba)  and  has  rumination, uterotonic, hemostatic and anti-inflammatory effects.  Therapeutic  doses  for  cows  were tested, which for rats amounted to 0.2 ml per head.  The  phytopreparation  was  adminitered subcutaneously three times, every other day. The control group rats were subcutaneously injected with a saline solution of sodium  chloride  (NaCI  0.9%)  according  to  the same  scheme.  The  methods  of  digital  microscopy were used  in the  study of micropreparations.  Morphometric  analysis  of  the histoarchitectonics  of  the  uterus  revealed  a statistically significant increase in the diameter of the cross-section of the uterus of female  rats  of  the  experimental  group  by 25.8%  (p≤0.05) compared  with  the  control group due to an increase in the epithelial by 24.5% (p≤0.05) and the endometrial lamina of  the  uterine  mucosa  by  32%  (p≤0.05), which indicates on increasing the secretory activity of the epithelial cover of the uterus. In  the  experimental  group  of  rats  treated with phytopreparation, a significant increase of 46% (p<0.05) in the number of primordial follicles was noted. There was also a tendency to increase the number of primary – by 36.3%, secondary – by 41.7%, tertiary – by 75%  of  follicles  and  yellow  bodies –  by 22.2%  in  rats  of  the  experimental  group compared with the control indicators.  Quantitative indicators of the follicular composition of the ovaries of rats of the experimental group and an increase in  the follicle aging coefficient by 60% (p≤0.05) indicate stimulation  of  the  ovarian  reserve  against  the background of the use of phytopreparation.