Prognostic assessment of the quality of oocytes in mares used in ART programs based on biochemical parameters of follicular fluid and blood serum

Abstract

The biochemical composition of the mare’s follicle fluid was studied, and the level of identity of this composition with the biochemical parameters of their blood was established. It has been shown that the follicular fluid (FF) of large follicles (≥37 mm) contains significantly more electrolytes – Na, K, P and serum Fe, but significantly less total protein, uric acid, cholesterol, triglycerides, HDL cholesterol and intracellular enzymes - ALT, AST, CPK, LDH, GGTP, alkaline phosphatase, than in blood serum (p<0.05). At the same time, no significant differences were found in the content of glucose, urea, magnesium, albumin, all types of bilirubin, creatinine, LDL cholesterol, alphaamylase, and calcium in the studied media. The presence of correlations has been shown between the quantitative indicators of most components of the biochemical composition of the FF and blood serum, and in the FF itself - between the components of the lipid profile with Ca, P, glucose, and albumin. Significant relationships were also established between the content of serum Fe, Mg, Ca, total protein, urea, cholesterol, triglycerides, intracellular enzymes - ALT, AST, CPK, LDH, GGTP, alkaline phosphatase, bilirubin and creatinine in the FF and blood serum (p<0. 05). The obtained statistically reliable experimental data can be used for prognostic assessment of the composition of follicular fluid and the corresponding quality of oocytes based on biochemical analysis of blood serum in mares. The statistically reliable experimental data obtained can be used for a prognostic assessment of the composition of follicular fluid and the corresponding quality of oocytes by biochemical analysis of blood serum of mares.