HORMONAL PROFILE OF FOLLICLE FLUID OF DIFFERENT DIAMETERS IN RELATION TO VIABILITY AND LIPID CONTENT IN GRANULOSA CELLS OF SUS SCROFA DOMESTICUS

Abstract

Modeling of oocyte maturation systems in farm animals is based on the available information on the composition of follicular fluid (FF), however, there are no data on the hormonal status of porcine follicular fluid in relation to the viability and functional state of the lipidome in granulosa cells (GCs) in the dynamics of folliculogenesis. We analyzed the hormonal profile (LH, FSH, T3, T4, cortisol) of porcine antral follicle fluid and obtained data on the effect of these hormones on the viability and lipid content (lipid droplets-LDs) of porcine GCs in follicles of different diameters (ø <3 mm, ø 3-5 mm and ø>5 mm). An inverse correlation was found between the proportion of GCs with high fluorescence intensity of the lipid droplet complex – NileRed (IFNileRed/LD - a marker of lipid droplets content) and the concentration of cortisol in the dynamics of folliculogenesis (-0.830, p<0.05), as well as an inverse correlation between the cortisol content and the proportion of viable cells (- 0.995, p<0.05). A direct correlation was found between the content of both T3 and T4 with IFNileRed/LD (0.901, 0.946, p<0.05) in GCs, which probably suggests accumulation of the energy substrate in the cytosol. There were no reliable correlation values in the content of LH in the FF and the proportion of GCs with high IFNileRed/LD intensity. The high level of correlation in the concentration of T3, T4 in the FF and cortisol with the lipid’s content in the GCs indicates a significant influence of these hormones on the energy reserve and viability of the GCs during folliculogenesis and allows us to consider them as potential candidates for an indepth study of the mechanisms of metabolic disorders that provoke abnormalities in the development of offspring obtained at in vitro maturation and fertilization of female gametes, as well as for modeling the composition of the media for the formation of a mature porcine ovum in vitro.