Analysis of the Worldwide Diversity of Xanthomonas hortorum pv. carotae, the Agent of Bacterial Blight of Carrot, Reveals Two Distinct Populations

Abstract

Xanthomonas hortorum pv. carotae is a pathogen responsible for the bacterial blight of carrot. Although it infects carrot fields worldwide, its diversity remains unknown. Here, we validated by PCR the identification as X. hortorum pv. carotae of most of the strains isolated from symptomatic carrots. We studied their diversity by sequencing seven housekeeping genes. The analysis confirmed the identity of most of the strains previously identified as X. hortorum pv. carotae and highlighted the presence of two clusters inside the pathovar carotae. The 18 non- X. hortorum pv. carotae have been identified as Xanthomonas from other species. As the X. hortorum pv. carotae strains clustered only in two groups by this analysis, a new multilocus variable number of tandem repeats analysis scheme was developed. It allowed us to validate the subclustering in two groups and observe diversity among them. At the same time, based on this study and data from the literature, we proposed the strain CFBP 7900 as the neopathotype strain of the pathovar. The strain should replace the holopathotype strain CFBP 4997, as it is no longer available in international collections. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .