Author:
Zhao Yu,Chen Xiyao,Ma Lei,Zuo Zhiyi,Zhu Zhenghua,Zhu Xiaoling,Wang Qiang,He Ertao,Xiong Lize,Pei Jianming,Xu Lixian,Hou Lihong,Chen Shaoyang
Abstract
Abstract
Background
Electroacupuncture (EA) pretreatment can induce the tolerance against focal cerebral ischemia. However, the underlying mechanisms have not been fully understood. Emerging evidences suggest that canonical Notch signaling may be involved in ischemic brain injury. In the present study, we tested the hypothesis that EA pretreatment-induced tolerance against focal cerebral ischemia is mediated by Notch signaling.
Results
EA pretreatment significantly enhanced Notch1, Notch4 and Jag1 gene transcriptions in the striatum, except Notch1 intracellular domain level, which could be increased evidently by ischemia. After ischemia and reperfusion, Hes1 mRNA and Notch1 intracellular domain level in ischemic striatum in EA pretreatment group were increased and reached the peak at 2 h and 24 h, respectively, which were both earlier than the peak achieved in control group. Intraventricular injection with the γ-secretase inhibitor MW167 attenuated the neuroprotective effect of EA pretreatment.
Conclusions
EA pretreatment induces the tolerance against focal cerebral ischemia through activation of canonical Notch pathway.
Publisher
Springer Science and Business Media LLC
Subject
Cellular and Molecular Neuroscience,General Neuroscience
Cited by
21 articles.
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