Author:
Chen Sining,Li Dandan,Zeng Zhipeng,Zhang Wei,Xie Hongliang,Tang Jianming,Liao Shengyou,Cai Wanxia,Liu Fanna,Tang Donge,Dai Yong
Abstract
Abstract
Purpose
Oral adenoid cystic carcinoma (OACC) has high rates of both local–regional recurrence and distant metastasis. The objective of this study is to investigate the impact of Khib on OACC and its potential as a targeted therapeutic intervention.
Experimental design
We investigated the DEPs (differentially expressed proteins) and DHMPs between OACC-T and OACC-N using LC–MS/MS-based quantitative proteomics and using several bioinformatics methods, including GO enrichment analysis, KEGG pathway analysis, subcellular localization prediction, MEA (motif enrichment analysis), and PPI (protein–protein interaction networks) to illustrate how Khib modification interfere with OACC evolution.
Results
Compared OACC-tumor samples (OACC-T) with the adjacent normal samples (OACC-N), there were 3243 of the DEPs and 2011 Khib sites were identified on 764 proteins (DHMPs). DEPs and DHMPs were strongly associated to glycolysis pathway. GAPDH of K254, ENO of K228, and PGK1 of K323 were modified by Khib in OACC-T. Khib may increase the catalytic efficiency to promote glycolysis pathway and favor OACC progression.
Conclusions and clinical relevance
Khib may play a significant role in the mechanism of OACC progression by influencing the enzyme activity of the glycolysis pathway. These findings may provide new therapeutic options of OACC.
Funder
Postdoctoral Science Foundation of China
General Project of Basic Research of Shenzhen Natural Science Foundation
Publisher
Springer Science and Business Media LLC
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