Author:
Wang Jiaqi,Feng Jiajia,Jia Wei,Yuan Tingxun,He Xinyu,Wu Qianqian,Peng Fujun,Gao Wei,Yang Zhongfa,Tao Yuanyong,Li Qian
Abstract
Abstract
Background
Corynebacterium pyruviciproducens is a recently described species of Corynebacterium. There are few reports on the microbiological characteristics of the new species, and there is a lack of reports on the genomic analysis of the species.
Results
This study involved a clinical isolate from the pus of a hospital patient with sebaceous gland abscesses. The clinically isolated strain was identified as C. pyruviciproducens strain WYJY-01. In this study, referring to Koch’s postulates, we observed the pathological changes of animal models infected by intraperitoneal injection and subcutaneous injection of pure culture of the strain WYJY-01. Furthermore, the strain WYJY-01 was isolated and cultured again from animal models' subcutaneous abscess drainage fluid. Subsequently, the genomics of the strain WYJY-01 was analyzed. By comparing various gene databases, this study predicted the core secondary metabolite gene cluster of the strain WYJY-01, virulence factor genes carried by prophage, pathogenicity islands, and resistance islands. In addition, the genomes of C. pyruviciproducens strain WYJY-01, ATCC BAA-1742 T, and UMB0763 were analyzed by comparative genomics, and the differential genes of strain WYJY-01 were compared, and their functions were analyzed.
Conclusion
The findings showed that the strain WYJY-01 had pathogenicity, supplementing the phenotype characteristics of C. pyruviciproducens. Meanwhile, this research revealed the possible molecular mechanism of the pathogenicity of the strain WYJY-01 at the gene level through whole genome sequence analysis, providing a molecular basis for further research.
Funder
the Tai-Shan Scholar Program from Shandong Province
Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China
the National Natural Science Foundation of China
the Shandong Provincial Natural Science Foundation
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology