Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis
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Published:2023-08-10
Issue:1
Volume:12
Page:
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ISSN:2049-9957
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Container-title:Infectious Diseases of Poverty
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language:en
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Short-container-title:Infect Dis Poverty
Author:
Shumuye Nigus Abebe, Li Li, Li Wen-Hui, Zhang Nian-Zhang, Wu Yan-Tao, Wu Yao-Dong, Tian Wen-Jun, Zhang Lin-Sheng, Nian Xiao-Feng, Dai Guo-Dong, Chen Wei-Gang, Gao Sheng-Zhi, Tian Xue-Qi, Liu Jun-Shi, Li Bin, Kebede Nigatu, Fu Bao-Quan, Yan Hong-Bin, Jia Wan-ZhongORCID
Abstract
Abstract
Background
In the normal life cycle of the parasite (Echinococcus multilocularis) that causes alveolar echinococcosis, domestic and wild carnivores act as definitive hosts, and rodents act as intermediate hosts. The presented study contributes to the research on the distribution and transmission pattern of E. multilocularis in China having identified sheep as an unusual intermediate host taking part in the domestic transmission of alveolar echinococcosis in Gansu Province, China.
Methods
From 2020 to 2021, nine whitish different cyst-like were collected from the liver of sheep in Gansu Province for examination. A near complete mitochondrial (mt) genome and selected nuclear genes were amplified from the cyst-like lesion for identification. To confirm the status of the specimen, comparative analysis with reference sequences, phylogenetic analysis, and network analysis were performed.
Results
The isolates displayed ≥ 98.87% similarity to E. multilocularis NADH dehydrogenase sub-unit 1 (nad1) (894 bp) reference sequences deposited in GenBank. Furthermore, amplification of the nad4 and nad2 genes also confirmed all nine samples as E. multilocularis with > 99.30% similarity. Additionally, three nuclear genes, pepck (1545 bp), elp-exons VII and VIII (566 bp), and elp-exon IX (256 bp), were successfully amplified and sequenced for one of the isolates with 98.42% similarity, confirming the isolates were correctly identified as E. multilocularis. Network analysis also correctly placed the isolates with other E. multilocularis.
Conclusions
As a result of the discovery of E. multilocularis in an unusual intermediate host, which is considered to have the highest zoonotic potential, the result clearly demonstrated the necessity for expanded surveillance in the area.
Graphical Abstract
Funder
National Key Research and Development Program Cultivation of Achievements of State Key Laboratory of Veterinary Etiological Biology NBCITS Central Public-Interest Scientific Institution Basal Research Fund Gansu Provincial Major Project for Science and Technology Development
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Public Health, Environmental and Occupational Health,General Medicine
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