Author:
Zhang Rusheng,Fu Huiyuan,Luo Can,Huang Zheng,Pei Ruiqing,Di Yu,Zhu Caiying,Peng Jiayi,Hu Huiqi,Chen Shan,Chen Jingfang,Chen Lamei,Xu Mingzhong,Yang Xuewen,Yang Rengui
Abstract
Abstract
Background
We investigated the presence of Chlamydia psittaci in poultry and the environment in live poultry wholesale markets in Changsha during 2021–2022 and conducted a phylogenetic analysis to understand its distribution in this market.
Methods
In total, 483 samples were analyzed using real-time polymerase chain reaction and 17 C. psittaci-positive samples using high-throughput sequencing, BLAST similarity, and phylogenetic analysis.
Results
Twenty-two out of 483 poultry and environmental samples were positive for C. psittaci (overall positivity rate: 4.55%) with no difference in positivity rates over 12 months. Chlamydia psittaci was detected at 11 sampling points (overall positivity rate: 27.5%), including chicken, duck, and pigeon/chicken/duck/goose shops, with pigeon shops having the highest positivity rate (46.67%). The highest positivity rates were found in sewage (12.5%), poultry fecal (7.43%), cage swab (6.59%), avian pharyngeal/cloacal swab (3.33%), and air (2.29%) samples. The ompA sequences were identified in two strains of C. psittaci, which were determined to bear genotype B using phylogenetic analysis. Thus, during monitoring, C. psittaci genotype B was detected in the poultry and environmental samples from the poultry wholesale market in Changsha.
Conclusions
To address the potential zoonotic threat, C. psittaci monitoring programs in live poultry markets should be enhanced.
Funder
Hunan Provincial Natural Science Foundation of China
Scientific Research Project of Hunan Provincial Health Commission
Natural Science Foundation of Changsha
Publisher
Springer Science and Business Media LLC
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