Author:
Yin Yingxian,Li Jiahui,Su Ling,Ou Zhiying,Lv Qingqun,Xiao Misi,Wang Changbing,Zeng Dan,Gu Yiling,Yang Fengxia,Chen Minxia,Feng Shaojuan,Hu Wanming,Bu Fengling,Zhu Bing,Xu Yi
Abstract
Abstract
Background
Herpes simplex virus type 1 (HSV-1) infection is a common viral disease that mainly causes oral lesions, but can also cause genital lesions in some instances. Current treatments with nucleoside analogs are limited by the emergence of drug resistance. Therefore, novel anti-HSV-1 drugs are urgently needed.
Methods
In this study, we screened a library of 2080 compounds for anti-HSV-1 activity using a plaque formation assay. We selected 11 potential inhibitors of HSV-1 and further evaluated their antiviral effects by plaque reduction assay and real-time polymerase chain reaction (qPCR).
Results
Five compounds, namely ginsenoside Rd, brassinolide, rosamultin, 3’-hydroxy puerarin, and clinafloxacin HCl, showed potent anti-HSV-1 activity and completely suppressed plaque formation at a concentration of 10 µM. Among them, clinafloxacin HCl, a fluoroquinolone antibiotic, exhibited a high selectivity index for HSV-1.
Conclusions
Our findings suggest that these five compounds have potential antiviral properties against HSV-1 and may have different mechanisms of action. Further studies are warranted to elucidate the antiviral mechanisms of these compounds and to explore their therapeutic potential for HSV-1 infection.
Funder
Medical Scientific Research Foundation of Guangdong Province of China
Plan on enhancing scientific research in GMU
National Key Research and Development Program of China
Guangzhou Women and Childrenʼs Medical Center/Guangzhou Institute of Pediatrics
Publisher
Springer Science and Business Media LLC