Author:
Yu Yue,Xu Zhihua,Zhou Hao,Xu Ruyan,Xu Jia,Liu Wenjun,Wu Yuxin,Qiu Yue,Zhang Guangbo,Huang Xue,Chen Yan
Abstract
Abstract
Background
Increasing evidence proves that RBP7 plays a significant role in breast cancer (BC). The present study was aimed to investigate the mechanism of RBP7.
Methods
Western Blotting and qRT-PCR were performed for evaluating the expression levels. CCK8, colony forming, xenograft mouse model, wound healing and transwell assays were conducted to examine cell ability of proliferation, invasion and migration. Nile red staining and Oil red O staining were used for testing the lipid.
Results
RBP7 was related to overall survival (OS) in patients with HR + BC. RBP7 protein was significantly decreased in HR + BC tissues and cells. RBP7 suppressed HR + BC cell proliferation in vitro and in vivo, and inhibited migration and invasion. RBP7 reduced fatty acid in HR + BC cells by inhibiting the AKT/SREBP1 pathway.
Conclusions
RBP7 may function as a tumor suppressor in HR + BC by inhibiting the AKT/SREBP1 pathway and reducing fatty acid.
Funder
Suzhou Science & Technology plan project
National Natural Science Foundation of China
Natural Science Foundation project of the Training program
Natural Science Foundation of province of Jiangsu Province
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献