Wound healing potentials of herbal ointment containing Calendula officinalis Linn. on the alteration of immunological markers and biochemical parameters in excision wounded animals

Abstract

AbstractObjectiveThe present study was designed to investigate the in vivo wound healing activity of herbal ointment prepared fromCalendula officinalisLinn. on excision wounded rats.Materials and methodsThe excision wound model was employed for wound healing activity in albino rats. Healthy albino rats (150–200 g) of either sex were taken for excision wound model. Animals were divided into five groups of six animals in each. Group I served as normal control, Group II served as excision wounded control without treatment and Group III, IV served as excision wounded rats were treated with herbal ointment of two different doses (10% and 20%) applied topically for 14 days and group V served as excision wounded animals treated with reference ointment soframycin. Healing potential was evaluated by the rate of wound contraction, immunological markers like IL-6(Interleukin 6), TNF-alpha (Tumor necrosis factor -α), PDGF (Platelet Derived Growth Factor) and EGF (Epidermal Growth Factor), lipid peroxide (LPO), superoxide dismutase (SOD), and biochemical parameters like hydroxyproline, hesosamine, and tissue protein.ResultsThe topical application of herbal ointment treated groups showed increase in the levels of growth factors such as PDGF and EGF hydroxy proline, hesosamine, tissue protein, SOD and wound contraction and the ointment normalized the levels of lipid peroxide, IL-6, TNF-alpha compared than that of excision wounded animals.ConclusionFrom the above results, it was concluded that the topical application of herbal ointment exhibited significant wound healing activity in excision wounded rats as evidenced by increased wound contraction and collagen synthesis.