Author:
He Junmin,Zhao Bingru,Huang Xixia,Fu Xuefeng,Liu Guifen,Tian Yuezhen,Wu Cuiling,Mao Jingyi,Liu Jing,Gun Shuangbao,Tian Kechuan
Abstract
Abstract
Background
Merino sheep are the most famous fine wool sheep in the world. They have high wool production and excellent wool quality and have attracted worldwide attention. The fleece of the Merino sheep is composed predominantly of wool fibers grown from secondary wool follicles. Therefore, it is necessary to study the development of hair follicles to understand the mechanism of wool production. The hair follicle is a complex biological system involved in a dynamic process governed by gene regulation. The hair follicle development process is very complex and poorly understood. The purpose of our research is to identify candidate genes related to hair follicle development, provide a theoretical molecular breeding basis for the cultivation of fine wool sheep, and provide a reference for the problems of hair loss and alopecia areata that affect human beings.
Results
We analyzed mRNAs data in skin tissues of 18 Merino sheep at four embryonic days (E65, E85, E105 and E135) and two postnatal days (P7 and P30). G1 to G6 represent hair follicles developmental at six stages (i.e. E65 to P30). We identified 7879 differentially expressed genes (DEGs) and 12623 novel DEGs, revealed different expression patterns of these DEGs at six stages of hair follicle development, and demonstrated their complex interactions. DEGs with stage-specific expression were significantly enriched in epidermal differentiation and development, hair follicle development and hair follicle morphogenesis and were enriched in many pathways related to hair follicle development. The key genes (LAMA5, WNT10A, KRT25, SOSTDC1, ZDHHC21, FZD1, BMP7, LRP4, TGFβ2, TMEM79, SOX10, ITGB4, KRT14, ITGA6, and GLI2) affecting hair follicle morphogenesis were identified by network analysis.
Conclusion
This study provides a new reference for the molecular basis of hair follicle development and lays a foundation for further improving sheep hair follicle breeding. Candidate genes related to hair follicular development were found, which provided a theoretical basis for molecular breeding for the culture of fine wool sheep. These results are a valuable resource for biological investigations of fleece evolution in animals.
Publisher
Springer Science and Business Media LLC
Reference85 articles.
1. Sulayman A, Tian K, Huang X, Tian Y, Xu X, Fu X, et al. Genome-Wide Identification and Characterization of Long Non-Coding RNAs Expressed During Sheep Fetal and Postnatal Hair Follicle Development. Sci Rep. 2019;9(1):8501. https://doi.org/10.1038/s41598-019-44600-w.
2. Galbraith H. Fundamental Hair Follicle Biology and Fine Fibre Production in Animals. Animal. 2010;4(9):1490–509. https://doi.org/10.1017/S175173111000025X.
3. Hardy H, Lyne A. The Pre-Natal Development of Wool Follicles in Merino Sheep. Aust J Biol Sci. 1956;9(3):421–41.
4. Brook AH, Short BF, Lyne AG. Formation of New Wool Follicles in the Adult Sheep. Nature. 1960;185:51. https://doi.org/10.1038/185051a0.
5. Chapman RE, Hopkins PS, Thorburn GD. The Effects of Fetal Thyroidectomy and Thyroxine Administration on the Development of Skin and Wool Follicles of Sheep Fetuses. J Anat. 1974; 117(Pt 2):419–32. https://www.ncbi.nlm.nih.gov /pmc/articles/PMC1231415/pdf/janat00385–0188.
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献