Author:
Pongchaikul Pisut,Romero Roberto,Mongkolsuk Paninee,Vivithanaporn Pornpun,Wongsurawat Thidathip,Jenjaroenpun Piroon,Nitayanon Perapon,Thaipisuttikul Iyarit,Kamlungkuea Threebhorn,Singsaneh Arunee,Santanirand Pitak,Chaemsaithong Piya
Abstract
Abstract
Background
Preterm labor syndrome is associated with high perinatal morbidity and mortality, and intra-amniotic infection is a cause of preterm labor. The standard identification of causative microorganisms is based on the use of biochemical phenotypes, together with broth dilution-based antibiotic susceptibility from organisms grown in culture. However, such methods could not provide an accurate epidemiological aspect and a genetic basis of antimicrobial resistance leading to an inappropriate antibiotic administration. Hybrid genome assembly is a combination of short- and long-read sequencing, which provides better genomic resolution and completeness for genotypic identification and characterization. Herein, we performed a hybrid whole genome assembly sequencing of a pathogen associated with acute histologic chorioamnionitis in women presenting with PPROM.
Results
We identified Enterococcus faecium, namely E. faecium strain RAOG174, with several antibiotic resistance genes, including vancomycin and aminoglycoside. Virulence-associated genes and potential bacteriophage were also identified in this genome.
Conclusion
We report herein the first study demonstrating the use of hybrid genome assembly and genomic analysis to identify E. faecium ST17 as a pathogen associated with acute histologic chorioamnionitis. The analysis provided several antibiotic resistance-associated genes/mutations and mobile genetic elements. The occurrence of E. faecium ST17 raised the awareness of the colonization of clinically relevant E. faecium and the carrying of antibiotic resistance. This finding has brought the advantages of genomic approach in the identification of the bacterial species and antibiotic resistance gene for E. faecium for appropriate antibiotic use to improve maternal and neonatal care.
Funder
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Eunice Kennedy Shriver National Institute of Child Health and Human Development
Mahidol University
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
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