Author:
Zhao Wei,Liu Jingwen,Wu Jie,Ma Xiaozhou,Wang Xi,Zhang Leyu,Han Zhe,Yang Jianming,Cui Yameng,Hu Xin,Deng Jiayin
Abstract
Abstract
Background
Emerging transcriptome-wide high-throughput screenings reveal the landscape and functions of RNAs, such as circular RNAs (circRNAs), in human cancer. In addition, the post-transcriptional RNA internal modifications, especially N6-methyladenosine (m6A), greatly enrich the variety of RNAs metabolism. However, the m6A modification on circRNAs has yet to be addressed.
Results
Here, we report an epitranscriptome-wide mapping of m6A-modified circRNAs (m6A-circRNA) in oral squamous cell carcinoma (OSCC). Utilizing the data of m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) and m6A-circRNAs microarray, we found that m6A-circRNAs exhibited particular modification styles in OSCC, which was independent of m6A-mRNA. Besides, m6A modification on circRNAs frequently occurred on the long exons in the front part of the coding sequence (CDS), which was distinct from m6A-mRNA that in 3’-UTR or stop codon.
Conclusion
In conclusion, our work preliminarily demonstrates the traits of m6A-circRNAs, which may bring enlighten for the roles of m6A-circRNAs in OSCC.
Funder
National Natural Science Foundation of China
Science & Technology Development Fund of the Tianjin Education Commission for Higher Education
Hospital Project of Tianjin Medical University Cancer Institute and Hospital
Publisher
Springer Science and Business Media LLC
Cited by
6 articles.
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