Author:
Ning Junya,Liu Min,Shen Jing,Wang Deping,Gao Lijuan,Li Huiyu,Cao Jimin
Abstract
AbstractColorectal cancer (CRC) is one of the malignant tumors with the highest morbidity and mortality and poor prognosis. The mammalian gene family of Cab45/reticulocalbin/ERC-45/calumenin (CREC) consists of RCN1, RCN2, RCN3, SDF4 and CALU. Although CREC family members have been associated with CRC, the expression pattern, prognostic value, and the role of CREC family in CRC remain unclear. In this study, the expression, survival and biological functions of CREC family in CRC were determined via bioinformatic datasets analysis and experimental verification on clinical CRC specimen. Bioinformatic analysis showed that the expression levels of most CREC family genes were higher in CRC tissues than in normal colorectal tissues. The qPCR and western blot results also revealed that the transcriptional and protein levels of CREC family were elevated in CRC tissues compared with adjacent tissues. Besides, CREC family was significantly correlated with advanced tumor stage and poor prognosis of CRC patients. The expression levels of CREC family had correlations with genomic mutation and methylation, and with the infiltration levels of CD4 + T cells, macrophages, neutrophils, and dendritic cells in the microenvironment of CRC. Functional networks enrichment analysis indicated that the genes of CREC family were essential factors for CRC metastasis. Collectively, these findings suggest that CREC family might be potential targets for the treatment of CRC and candidate prognostic markers for CRC patients.
Funder
Basic Research Program of Shanxi Province
Doctor of Shanxi Medical University
Science Research Start-up Fund for Doctor of Shanxi Province
National Natural Science Foundation of China
Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi
Shanxi Province “136” Revitalization Medical Project Construction Funds
Key Medical Science and Technology Program of Shanxi Province
Shanxi “1331 Project” Quality and Efficiency Improvement Plan
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Genetics,Oncology