Mechanochemical-assisted extraction and enzymatic hydrolysis of calcium phytate from defatted rice bran

Author:

Zheng Lei,Zhu XingYi,Wang Jialu,Su Weike

Abstract

Abstract Background Mechanochemical-assisted extraction (MCAE) and mechanochemical-assisted enzyme catalysis (MCAEC) methods were developed for extraction and enzymatic hydrolysis of calcium phytate from defatted rice bran, respectively. Results The MCAE conditions were optimized by response surface methodology: 10% sodium carbonate as the solid reagent, 33 min grinding time, 18:1 ball–material ratio, 330 rpm ball milling speed, and pH 5.5 extraction. Under these conditions, the yield of calcium phytate was 97.0 mg/g. The MCAEC conditions were optimized as follows: 2% enzyme addition, MM400 milling time 36 min, PM400 milling speed 262 rpm, MM400 milling frequency 27 Hz, and the addition of pH 5.5 sodium acetate buffer 3 mL. Under these conditions, the hydrolysis rate of calcium phytate was 39.7%. Conclusion Compared with traditional extraction and enzymatic hydrolysis methods, MCAE and MCAEC could obtain higher yields and hydrolysis rates, respectively. Graphical Abstract

Funder

the Huadong Medicine Joint Funds of Zhejiang Provincial Natural Science Foundation of China

the Basic Public Welfare Research Project of Zhejiang Province

the National Natural Science Foundation of China

Publisher

Springer Science and Business Media LLC

Subject

Agronomy and Crop Science,Biochemistry,Food Science,Biotechnology

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