Author:
Wu Yu,Li Wei,Zhou Qingfeng,Li Qunhui,Xu Zhichao,Shen Hanqin,Chen Feng
Abstract
Abstract
Background
Porcine epidemic diarrhea virus (PEDV) has caused enormous economic losses to the global pig industry. Currently available PEDV vaccine strains have limited protective effects against PEDV variant strains.
Methods
In this study, the highly virulent epidemic virus strain CT was serially passaged in Vero cells for up to 120 generations (P120). Characterization of the different passages revealed that compared with P10 and P64, P120 had a higher viral titer and more obvious cytopathic effects, thereby demonstrating better cell adaptability.
Results
Pathogenicity experiments using P120 in piglets revealed significant reductions in clinical symptoms, histopathological lesions, and intestinal PEDV antigen distribution; the piglet survival rate in the P120 group was 100%. Furthermore, whole-genome sequencing identified 13 amino acid changes in P120, which might be responsible for the attenuated virulence of P120.
Conclusions
Thus, an attenuated strain was obtained via cell passaging and that this strain could be used in preparing attenuated vaccines.
Funder
Provincial Agricultural Science and Technology Innovation and Extension Project 2017
Guangdong Agricultural Department Project
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
Reference35 articles.
1. Lee C. Porcine epidemic diarrhea virus: an emerging and reemerging epizootic swine virus. Virol J. 2015;12:93.
2. Lin H, Chen L, Gao L, Yuan X, Ma Z, Fan H. Epidemic strain YC2014 of porcine epidemic diarrhea virus could provide piglets against homologous challenge. Virol J. 2016;13:68.
3. Park S, Kim S, Song D, Park B. Novel porcine epidemic diarrhea virus variant with large genomic deletion, South Korea. Emerg Infect Dis. 2014;20:2089.
4. Sun D, Wang X, Wei S, Chen J, Feng L. Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review. J Vet Med Sci. 2016;78:355–63.
5. Liu X, Zhang Q, Zhang L, Zhou P, Yang J, Fang Y, et al. A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate. Virus Res. 2019;259:18–27.
Cited by
13 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献