Author:
Zhang Ming,He Zuping,Wen Lixin,Wu Jing,Yuan Liyun,Lu Yin,Guo Chengzhi,Zhu Li,Deng Sijun,Yuan Hui
Abstract
Abstract
Objective
Very little information is known about the toxic effects of cadmium on somatic cells in mammalian testis. The objective of this study is to explore the toxicity of cadmium on piglet Sertoli cells.
Methods
Sertoli cells were isolated from piglet testes using a two-step enzyme digestion and followed by differential plating. Piglet Sertoli cells were identified by oil red O staining and Fas ligand (FasL) expression as assayed by immunocytochemistry and expression of transferrin and androgen binding protein by RT-PCR. Sertoli cells were cultured in DMEM/F12 supplemented with 10% fetal calf serum in the absence or presence of various concentrations of cadmium chloride, or treatment with p38 MAPK inhibitor SB202190 and with cadmium chloride exposure. Apoptotic cells in seminiferous tubules of piglets were also performed using TUNEL assay in vivo.
Results
Cadmium chloride inhibited the proliferation of Piglet Sertoli cells as shown by MTT assay, and it increased malondialdehyde (MDA) but reduced superoxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) activity. Inhibitor SB202190 alleviated the proliferation inhibition of cadmium on piglet Sertoli cells. Comet assay revealed that cadmium chloride caused DNA damage of Piglet Sertoli cells and resulted in cell apoptosis as assayed by flow cytometry. The in vivo study confirmed that cadmium induced cell apoptosis in seminiferous tubules of piglets. Transmission electronic microscopy showed abnormal and apoptotic ultrastructure in Piglet Sertoli cells treated with cadmium chloride compared to the control.
Conclusion
cadmium has obvious adverse effects on the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis, and aberrant morphology. This study thus offers novel insights into the toxicology of cadmium on male reproduction.
Publisher
Springer Science and Business Media LLC
Subject
Developmental Biology,Endocrinology,Reproductive Medicine,Obstetrics and Gynaecology
Reference48 articles.
1. Kim J, Soh J: Cadmium-induced apoptosis is mediated by the translocation of AIF to the nucleus in rat testes. Toxicol Lett. 2009, 188: 45-51. 10.1016/j.toxlet.2009.03.006.
2. de Souza Predes F, Diamante MA, Dolder H: Testis response to low doses of cadmium in Wistar rats. Int J Exp Pathol. 2010, 91: 125-131. 10.1111/j.1365-2613.2009.00692.x.
3. Viswanadh EK, Rao BN, Rao BS: Antigenotoxic effect of mangiferin and changes in antioxidant enzyme levels of Swiss albino mice treated with cadmium chloride. Hum Exp Toxicol. 2010, 29: 409-418. 10.1177/0960327110361752.
4. Nicholson JK, Higham DP, Timbrell JA, Sadler PJ: Quantitative high resolution 1H NMR urinalysis studies on the biochemical effects of cadmium in the rat. Mol Pharmacol. 1989, 36: 398-404.
5. Griffin JL, Walker LA, Shore RF, Nicholson JK: Metabolic profiling of chronic cadmium exposure in the rat. Chem Res Toxicol. 2001, 14: 1428-1434. 10.1021/tx015521u.
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